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biotinylated recombinant cona  (GALAB Technologies GmbH)
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GALAB Technologies GmbH biotinylated recombinant cona
100 μg TconTS1 and TconTS2 expressed in CHO-Lec1 cells were incubated without (-) or with (+) 4000 units EndoH f glycosidase under native (-) or denaturing (+) conditions as described under Methods. A: 10% SDS polyacrylamide gel with subsequent Coomassie Brilliant Blue staining. B: Western blot of deglycosylated TconTS, detected using anti- Strep -tag mAb. C: Concanavalin A <t>(ConA)</t> lectin blot using 2 μg/mL <t>biotinylated</t> ConA and an peroxidase conjugated avidin-biotin system (ABC-Kit, VECTASTAIN) for detection. 50 ng TconTS sample were used for ConA and Western blot analysis and 800 ng for SDS-PAGE.
Biotinylated Recombinant Cona, supplied by GALAB Technologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated recombinant cona/product/GALAB Technologies GmbH
Average 90 stars, based on 1 article reviews
biotinylated recombinant cona - by Bioz Stars, 2026-02
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100 μg TconTS1 and TconTS2 expressed in CHO-Lec1 cells were incubated without (-) or with (+) 4000 units EndoH f glycosidase under native (-) or denaturing (+) conditions as described under Methods. A: 10% SDS polyacrylamide gel with subsequent Coomassie Brilliant Blue staining. B: Western blot of deglycosylated TconTS, detected using anti- Strep -tag mAb. C: Concanavalin A (ConA) lectin blot using 2 μg/mL biotinylated ConA and an peroxidase conjugated avidin-biotin system (ABC-Kit, VECTASTAIN) for detection. 50 ng TconTS sample were used for ConA and Western blot analysis and 800 ng for SDS-PAGE.

Journal: PLoS Neglected Tropical Diseases

Article Title: Carbohydrate Recognition Specificity of Trans-sialidase Lectin Domain from Trypanosoma congolense

doi: 10.1371/journal.pntd.0004120

Figure Lengend Snippet: 100 μg TconTS1 and TconTS2 expressed in CHO-Lec1 cells were incubated without (-) or with (+) 4000 units EndoH f glycosidase under native (-) or denaturing (+) conditions as described under Methods. A: 10% SDS polyacrylamide gel with subsequent Coomassie Brilliant Blue staining. B: Western blot of deglycosylated TconTS, detected using anti- Strep -tag mAb. C: Concanavalin A (ConA) lectin blot using 2 μg/mL biotinylated ConA and an peroxidase conjugated avidin-biotin system (ABC-Kit, VECTASTAIN) for detection. 50 ng TconTS sample were used for ConA and Western blot analysis and 800 ng for SDS-PAGE.

Article Snippet: Concanavalin A (ConA), Sepharose and biotinylated recombinant ConA were purchased from Galab, Germany.

Techniques: Incubation, Staining, Western Blot, Strep-tag, Avidin-Biotin Assay, SDS Page